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Home » Life Science » Products » Molecular Reagents » Isothermal Amplification

Isothermal Amplification

Isothermal amplification (including HAD, MCA, and LAMP) is a DNA amplification technique that has played an increasingly important role in point-of-care (POC) diagnostics. It offers several advantages over qPCR and other amplification techniques in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions. Isothermal amplification assays have been used for numerous applications including the detection of pathogens such as salmonella and malaria, GM crop contamination, and forensics to specifically detect human DNA.

Loop mediated isothermal amplification LAMP, for example, uses four to six primers that recognize several specific regions in the target DNA. The reaction is initiated by a strand-displacement polymerase such as Bst DNA polymerase and two specially designed primers which form loop structures to facilitate subsequent rounds of amplification producing high levels of DNA. LAMP reactions are very robust and inhibitor-tolerant, producing extremely large amounts of DNA that can be visualized by the eye (e.g. as a precipitate or color change). Specifically, 1 to 10 copies of DNA can be amplified to 109 to 1010 copies within 30 minutes, producing assays with excellent sensitivity and specificity.

For even greater tolerance, Meridian has also developed sample specific inhibitor tolerant LAMP mixes, designed to be used for direct amplification from crude lysates or inhibitor-rich samples, with the ability to tolerate even higher levels of inhibitors present in samples. In addition these mixes can be used as wet mixes or dried by lyophilization or air-drying for room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume, making them ideal for POCT development where minimal sample processing and fast turn-around times are essential.

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Name Specimen Type Application Catalog Order a Sample Sizes Description Wet(Liquid) Air-Dryable Dryable Glycerol-Free Lyo-Ready Concentration High Concentration Detail Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Glycerol-Free RNase H (HC) Reverse Transcriptase, NGS Enzymes, NASBA RT-qPCR MDX202 Order a Sample Available in 100 µL (2,500 Unit) or 250 µL (12,500 Unit) aliquots

Glycerol-free, high concentration RNA-specific endonuclease that hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA. Ideal for lyophilization.

 Yes Requires excipients Requires excipients YesYes Requires excipients 50 U/µL Yes View Details COA/Test Release Product Handling Guide
Glycerol-Free T7 RNA Polymerase (HC) NASBA NASBA MDX201 Order a Sample Available in 50,000 Unit or 500,000 Unit aliquots

Glycerol-free, high concentration DNA-dependant RNA Polymerase that requires a T7 promoter sequence, it an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications. Ideal for lyophilization.

 Yes Requires excipients Requires excipients YesYes Requires excipients 1,000 U/µL Yes View Details COA/Test Release Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Urine, 4x Urine, LAMP Mixes RT-LAMP MDX155 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

 Yes No Lyo-Ready YesYes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA LAMP Urine, 4x Urine, LAMP Mixes LAMP MDX154 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% urine. Perfect for multiplexing and fast cycling conditions. Used in the detection of urinary track infections, STD, tropical diseases, cancer screening, bladder and prostate cancer.

 Yes No Lyo-Ready YesYes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA LAMP Blood, 4x Blood, Plasma, Serum, LAMP Mixes LAMP MDX124 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% whole blood, 20% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

 Yes No Lyo-Ready YesYes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Stool, 4x Stool, LAMP Mixes RT-LAMP MDX145 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 5% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

 Yes No Lyo-Ready YesYes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA LAMP Stool, 4x Stool, LAMP Mixes LAMP MDX144 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 20% stool, 2 mg/mL bile salt and 40% Cary Blair media. Perfect for multiplexing and fast cycling conditions. Used in the detection of IBD, cancer, fecal microbiota and bacterial and viruses.

 Yes No Lyo-Ready YesYes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Saliva, 4x Saliva, Sputum, LAMP Mixes RT-LAMP MDX135 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

 Yes No Lyo-Ready YesYes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct DNA LAMP Saliva, 4x Saliva, Sputum, LAMP Mixes LAMP MDX134 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 60% crude saliva, sputum or mucin samples and 50% UTM. Perfect for multiplexing and fast cycling conditions. Used in the detection of a broad range of diseases, including cancer, respiratory viruses and bacterial infections.

 Yes No Lyo-Ready YesYes Yes 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready Direct RNA/DNA LAMP Blood, 4x Blood, Plasma, Serum, LAMP Mixes RT-LAMP MDX125 Order a Sample Available in 5 mL (1,000 Rxns) or 50 mL (10,000 Rxns) aliquots

Inhibitor tolerance to 10% whole blood, 10% plasma, compatible with all anticoagulants. Perfect for multiplexing and fast cycling conditions. Used in the detection of blood pathogens and liquid biopsy applications.

 Yes No Lyo-Ready YesYes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Air-Dryable DNA LAMP LAMP Mixes LAMP MDX119 Order a Sample Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots

Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays.

 Yes Yes Air-Dryable YesYes No 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Air-Dryable RT-LAMP 1-Step Mix, 4x LAMP Mixes RT-LAMP MDX118 Order a Sample Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots

Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and air-dryable excipients, ideal for developing ambient temperature, LAMP assays.

 Yes Yes Air-Dryable YesYes No 4x Yes View Details Safety Data Sheet COA/Test Release User Guide Product Handling Guide
Lyo-Ready RT-LAMP 1-Step Mix, 4x LAMP Mixes RT-LAMP MDX108 Order a Sample Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots

Glycerol-free mix for isothermal applications such as RT-LAMP, containing Bst DNA polymerase, reverse transcriptase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays.

 Yes No Lyo-Ready YesYes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Lyo-Ready LAMP Mix, 4x LAMP Mixes LAMP MDX097 Order a Sample Available in 5 mL (800 Rxns) or 50 mL (8,000 Rxns) aliquots

Glycerol-free mix for isothermal applications such as LAMP, containing Bst DNA polymerase, reaction buffer, dNTPs and lyo-compatible excipients, ideal for developing ambient temperature, LAMP assays.

 Yes No Lyo-Ready YesYes Yes 4x Yes View Details COA/Test Release User Guide Product Handling Guide
Enzyme Dilution Buffer, 10x (Glycerol free) Buffer, Bst Enzymes & Buffers PCR MDX080 Order a Sample Available in 5 mL or 100 mL aliquots

Glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration.

 Yes Yes Air-Dryable,Lyo-Ready YesYes Yes 10x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Enzyme Dilution Buffer, 1x Buffer, Bst Enzymes & Buffers PCR MDX078 Order a Sample Available in 5 mL or 100 mL aliquots

Glycerol containing1x dilution buffer, for the dilution of enzymes to reaction concentration.

 Yes No No NoNo No 1x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Bst Reaction Buffer, 10x Buffer, Bst Enzymes & Buffers LAMP MDX076 Order a Sample Available in 5 mL or 100 mL aliquots

Reaction Buffer optimized for use with Bst DNA Polymerase.

 Yes Requires excipients Requires excipients YesYes Requires excipients 10x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Bst DNA Polymerase Isothermal DNA Polymerase, Bst Enzymes & Buffers LAMP MDX012 Order a Sample Available in 1 mL or 10 mL aliquots

DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).

 Yes No No NoNo No 8 units/mL No View Details Safety Data Sheet COA/Test Release Product Handling Guide
Inhibitor-Tolerant Bst Buffer Buffer, Bst Enzymes & Buffers LAMP MDX019 Order a Sample Available in 2 mL or 100 mL aliquots

Inhibitor-Tolerant Bst Buffer is specially designed to overcome the inhibition present in samples in direct LAMP reactions.

 Yes Requires excipients Requires excipients YesYes Requires excipients 10x No View Details Safety Data Sheet COA/Test Release Product Handling Guide
High Conc. Glycerol-Free Bst Isothermal DNA Polymerase, Bst Enzymes & Buffers LAMP MDX018 Order a Sample Available in 80 µL or 800 µL aliquots

High concentration glycerol-free DNA polymerase (exo-), with strand-displacement properties. used for Isothermal DNA amplification such as LAMP (Loop-mediated Isothermal Amplification).

 Yes Requires excipients Requires excipients YesYes Requires excipients 100 units/mL Yes View Details Safety Data Sheet COA/Test Release Product Handling Guide

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